DOI LINK: https://doi.org/10.59671/mT7LJ

Paper ID:mT7LJ

Volume:58

Issue:12

Title:ACTIVITY OF AZOSPIRILLUM BRASILENSE 137 DIAZOTROPHS AND THEIR INFLUENCE ON SPRING WHEAT PLANTS

Abstract:The strain of nitrogen-fixing bacteria Azospirillum brasilense 137, isolated from the washed roots of spring wheat, forms on the solid synthetic malate medium (SM) whitish-beige roundish colonies, which, with aging, acquire folds, pearly lustre, and accumulate pink pigment; on solid NFb medium containing Congo Red colonies are small and red. It was shown that the growth parameters of Azospirillum brasilense 137 during cultivation on the liquid semi-synthetic and synthetic media differed significantly. For the cultivation of diazotrophs in a batch culture, a liquid semi-synthetic medium with molasses was chosen, the chemical composition of which ensures the maximum titre of the bacterial suspension. The number of colony-forming units was 3.3 billion by 24 hours of cultivation. Inoculation of spring wheat seeds with A. brasilense 137 bacterial suspension in an optimized growth medium increases the intensity of the initial growth of spring wheat and in the conditions of the field experiment provides a 16% increase in plant productivity compared to the control during 3 years.

Keywords:Azospirillum brasilense, semi-synthetic medium, spring wheat, yield.

Authors:Yu.O. Vorobei, O.V. Lohosha, S.F. Kozar, T.O. Usmanova

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