In this study, antimicrobial effects of Oregano essential oil on some of the most important spoiler and pathogenic bacteria of food including Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella enteritidis, Escherichia coli and Klebsiella numonia was investigated. Antimicrobial properties of Oregano essential oil were conducted using the agar diffusion method. To determine the minimum inhibitory concentration growth (MIC), we used the color reduction method as a sensitive and accurate method, and the color used was Resazurin. In determining the minimum bactericidal concentration (MBC), after reading the MIC result from well 1 to the next well, the MIC was removed and cultured in a BHI-agar medium. The results show that Oregano essential oil has antibacterial properties that can be used as a low-priced and accessible source for therapeutic use in some bacterial infections.
In Iran, Arasbaran area is a very rich zone which has most of Iran\'s herbaceous species including medicinal, ornamental and edible products. Most of the trees in this area are oak, kikem, crimson, barberry, wild pomegranate, wild apples, wild pears and raspberries, and cucumbers, along with fruits called Qara Qat (Iranian redcurrant), which is very tasty and sour, and are seasoned in the middle of summer.\nThe structures of anthocyanin water-soluble and polar derivatives which have a wide membrane bound vesicle in a cell’s cytoplasm can be seen in different colors in an expanded range of colors such as red, purple, blue or black.\nMetal cations chelation of some anthocyanins of [ACN-Mg2+/Al3+/Ga3+/ Sn2+/Cr3+/Fe3+] clusters in aqueous medium were investigated to discover the color fluctuation of different derivatives of these compounds in the low pH. Chelation of Mn+ by ACN also improved the color expression range of ACN in more different pH environments.\nThe difference of ∆HR among [ACN-Mg2+/Al3+/Ga3+/ Sn2+/Cr3+/Fe3+] cluster chelation has been discussed the double bonds and carbonyl groups through the chelation of B ring for Cy, Dp and Pt anthocyanins in gas phase and water medium that explains the stability and color of [ACN-Mg2+/Al3+/Ga3+/ Sn2+/Cr3+/Fe3+] cluster chelation of Cy, Dp, and Pt pigments in a weak acidic condition.\nIn this work, the ACNs of Cy,Dp and Pt with highest chelate in the active part of these structures by metal ions of Al3+/Ga3+/Cr3+/Fe3+ /Mg2+ produce a variety range of colors under acidic pH; as divalent Mg2+ has shown various physicochemical properties considering its optimized geometry optimization as the bond length of O Mg2+ ≈1.9 Å and the bond angle of O Mg2+ O ≈ 112o while it has been indicated the bond length of O M3+ ≈1.8 Å and bond angle of O M3+ O ≈ 120o for trivalent metal cations of Al3+/Ga3+/ Sn2+/Cr3+/Fe3+ with more electron rich metal ions caused shifts and hue changes in the stability of color and structure. \nMoreover, we have found that [ACN-Mg2+/Al3+/Ga3+/ Sn2+/Cr3+/Fe3+] cluster chelation is dependent on the position of active cites of labeled oxygen atoms and cations in these structures which transfer the charge of electrons in aromatic cyclic chains because of water polar medium in contrast to gas phase. The spin density and partial charges have been obtained by fitting the electrostatic potential to fixed charge of O+17 , O+16 , and O+7 cations for Cy-Mn+(31), Dp-Mn+ (32) and Pt-Mn+(35) , respectively based on the electrophilic groups of Cy, Dp and Pt anthocyanin pigments which unravel the activity and the stability of these compounds in the natural products.
The present experiment was designed to investigate the microbial diversity of the brick kiln soil and to check the activity and enzyme kinetics of amylase from the bacterial isolates. The bacterial colonies were isolated from soil samples through the serial dilution method. The bacterial isolates were identified through morphological and molecular analysis. The 16s ribosomal RNA sequences of the isolates IR-1, IR-2, IR-3, IR-8, and IR-9 showed high similarities with Bacillus tequilensis, Bacillus paramycoides, Proteus alimentorum, Bacillus wiedmannii, and Pseudomonas aeruginosa, respectively. All of the bacterial isolates showed a positive catalase activity except IR-9. Furthermore, the isolates showed variable antagonistic effects against different bacterial pathogens. All of the strains produced indole acetic acid (IAA), and the concentrations increased in the presence of tryptophan application. The isolates showed the amylase enzyme activity and maximum activity of isolates was achieved in 4% starch concentration. The IR-9 isolate showed the highest amylase activity of 5.9 U/ml. The Vmax values of the extracellular amylase from different bacterial isolates ranged between 12.90 - 50.00 IU ml-1. The lowest Km value of 6.33 mg starch was recorded for IR-8 and the maximum Kcat value of 2.50 min-1 was observed for IR-3. The amylase activity of the isolates was significantly affected by a range of different incubation time, temperature, and pH values. Further tests are required before the potential utilization of these isolates for amylase production, and in the biopesticide and biofertilizer applications.
Boron plays an important role as an essential micronutrient in both plants and animals. The application of H3BO3 Nps (nanoparticles) as a fertilizer increases the length of roots and shoots. The effect of H3BO3 Nps was analyzed on the germination of wheat seeds. These particles were prepared from pure boric acid by using the n-Hexane. The SEM analysis shows crystal like structure of smooth surface with diameter 10.6mm. XRD analysis confirms that the presence of single peak in XRD pattern symbolizes the purity of the sample. FTIR analysis of H3BO3 NPs shows the presence of different functional groups like O-H, C=C (alkenes), C=C (aromatic), C-H (bending), C-O, C-N and C-C. The solutions were made in different concentrations such as 0 millimoles (blank), 0.25, 0.50, 0.75 and 1.0millimoles of nanoparticles and pure boric acid separately. The seeds of wheat were grown in each level of solution in petri dishes separately. After the 6 days of observation, it was noted that the nanoparticles of boric acid and the level 1.0mM had more significant effect in all the attributes of wheat seedlings than pure boric acid and other levels.
Publishing is a grand finale for any research and the ultimate aspiration of every researcher. This paper is bringing some thoughts of a few participants to have a bird\'s eye view on the knowledge of publishing. This process has various levels of challenges in a different situation. Normally, any researcher has to go through the subsequent amount of waiting time. But, in the current pandemic COVID - 19, researchers are blessed with an opportunity to disseminate their knowledge quickly than the usual speed. Social distancing has opened up the virtual platform to carry on their work online and reduced the length of the waiting line. Participants in this study are scholars of Ph. D and are neutral in their attitude about publishing experience. 115 scholars were sent with questionnaires through email and 20 agreed to respond. This paper is trying to portray the possible constraints scholars may have gone through while publishing their article
Novel coronavirus raged unnoticed and so effortlessly, many countries across the globe found that the best way to ensure people to have minimal contact with each other by issuing total lockdown. The concept of lockdown is a social boon to minimize the spread but made life difficult and easy for individuals. The current study aimed at understanding the experiences of ten different participants such as a senior citizen, home maker, and work from home candidate and school going student. The data obtained was analysed by theme analysis. It was observed that bond and attachment were increased among family members, self-care was improved despite of the crisis faced by people. Also, it has brought happiness, health, safety, satisfaction and priority among individuals.
The effects of cell phone use on bike riders’ behaviour are studied. Most of bike riders confess using cellphone devices while driving. Moreover, relationship between close accidents and accidents has been found with the use of cell phone, reflecting a risk factor for bike riders. This study examines the relationship between such type of behaviours, comprising calling and manipulating the screen, and the frequency of close accidents and actual accidents among bike riders. We conducted a web based survey measuring cellphone-specific violations, errors, close accidents and accident to bike riders (N=289; age range; 18-60).We hypothesised that the relationship between cellphone use and close accidents would be explained by an increase in the number of errors committed, thus increasing the likelihood of being involved in close accidents. Moreover, we hypothesised that close accidents will predict actual accidents. Outcomes of path analysis showed that cellphone-specific violations predicted accidents throughout their consecutive effects on errors and close accidents only in the subsample of men. These findings offer an explanation of how cellphone use contributes to increase the likelihood of getting involved in close accidents and actual accidents. The current study builds a path model explaining how cellphone-specific violations lead to more close accidents among bike riders.”